Required programs: Install python (version 2.1 or newer) www.python.org Install Numeric Python numpy.sourceforge.net Install R www-r-project.org Program descriptions: ai2gc: read a clontech Atlas ImageArray text report and output a 2 column genecluster format file. gcs2r: read in several trials in genecluster format and output a csv file for R pairedtest: A shell script, chop the gcs2r csv file into individual genes, then run a paired t test on each gene, printing the significance test. Sample session: $ ai2gc trial1.txt > trial1.out $ ai2gc trial2.txt > trial2.out $ gcs2r trial1.out trial2.out > trials-1-2.out $ pairedtest trials-1-2.out GENE01 t = -0.8806, df = 1, p-value = 0.5404 GENE02 t = 0.3225, df = 1, p-value = 0.8014 GENE03 t = 0.8945, df = 1, p-value = 0.5354 GENE04 t = 6.5598, df = 1, p-value = 0.0963 GENE05 t = -0.9545, df = 1, p-value = 0.5148 GENE06 t = -0.7546, df = 1, p-value = 0.5884 GENE07 t = -0.1303, df = 1, p-value = 0.9175 GENE08 t = 1.0167, df = 1, p-value = 0.4947 GENE09 t = 0.0992, df = 1, p-value = 0.937 GENE10 t = 1.1561, df = 1, p-value = 0.454